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Related ArticlesP3X63Ag8 (小鼠骨髓瘤細(xì)胞) (種屬鑒定正確)源自P3K細(xì)胞株(源自漿細(xì)胞MOPC-21的組織培養(yǎng)株),P3X63Ag8能抗0.1mM 8-氮雜鳥嘌呤,但在HAT培養(yǎng)基中死亡。據(jù)報(bào)道,P3X63Ag8細(xì)胞是缺失了3-酮類固醇還原酶活性的膽固醇營(yíng)養(yǎng)缺陷型細(xì)胞。
CHO/dhFr- [CHO-DXB11] (倉(cāng)鼠卵巢細(xì)胞)為二氫葉酸還原酶缺陷細(xì)胞。在沒(méi)有HT(次黃-嘌呤-胸腺嘧啶)時(shí),CHO/dhFr-細(xì)胞會(huì)死亡;CHO/dhFr-細(xì)胞生長(zhǎng)培養(yǎng)基中加氨甲喋呤,可以防止對(duì)藥物低抗性的回變細(xì)胞的生長(zhǎng)。
NRK細(xì)胞源于Osborne-Mendel大鼠的正常腎組織。
SHZ-88細(xì)胞源自DMBA誘導(dǎo)的大鼠乳房癌。大多數(shù)SHZ-88細(xì)胞是上皮細(xì)胞樣的,也可以見到巨大細(xì)胞團(tuán)和病態(tài)有絲分裂的SHZ-88細(xì)胞;電鏡下可見SHZ-88細(xì)胞表面和橋粒上有微絲。第3天的有絲分裂指數(shù)是59%,移植到同源動(dòng)物中可以成活。
OKT 11細(xì)胞是一株用人的T細(xì)胞白血病細(xì)胞(急性淋巴細(xì)胞白血病)對(duì)小鼠進(jìn)行免疫,然后將免疫的小鼠脾細(xì)胞與P3X63Ag8.U1骨髓瘤細(xì)胞融合而建立的細(xì)胞系。免疫球蛋白;單克隆抗體;抗人T細(xì)胞(人T淋巴細(xì)胞)和人E玫瑰花環(huán)反應(yīng)陽(yáng)性胸腺細(xì)胞(人胸腺淋巴細(xì)胞);抗CD2。
“The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.